The research Tap Y-Adaptor objectives were to observe the growth and spore production of Metarhizium anisopliae grown at wheat-pollard and wheat-brand and to test the M.anisopliae pathogenicity on instar III rhinoceros beetle larva of coconut pest (Oryctes rhinoceros, L.).
The research were executed in 3 phases : (1) Regeneration phase of M.anisopliae, the experiment was arranged in single factor CRD with 3 kinds of wheat and 3 replications.The influences of the treatments on biomass, spore number and spore viability were observed then; (2) lethal dosage (LD50) determination phase; and (3) phatogenicity test of M.
anisopliae, the experiment was arranged in 3 x 3 factorial CRD, where the first factor was the kinds of wheat (i.e.Pollard, Brand and Pollard Brand) and the second factor was the dosage of biopesticide (i.
e.0,5 LD50 LD50 and 2 LD50).The phatogenicity testing was conducted to evaluate the effects on toxicity, mortality speed, and effication percentage.
The result indicated that the wheat-brand was the better medium for M.Anisopliae than the pollard and the pollard-brand ones.The average spore number (1021,67 x 1014 spora/ml), biomass (95,94 g) and the viability (127,00 x 108 spora/ml) were significantly higher than the others.
The lethal Pump Inlet Seal dosage obtained was 6,70g to gave the mortality percentage (73,33 %) and the effication percentage (73,33 %) significantly higher than the other treatments.